Ultra-fast DNA separations using capillary electrophoresis.
نویسندگان
چکیده
منابع مشابه
Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
Capillary electrophoresis arrays have been fabricated on planar glass substrates by photolithographic masking and chemical etching techniques. The photolithographically defined channel patterns were etched in a glass substrate, and then capillaries were formed by thermally bonding the etched substrate to a second glass slide. High-resolution electrophoretic separations of phi X174 Hae III DNA r...
متن کاملHigh speed DNA sequencing by capillary electrophoresis.
A major challenge of the Human Genome Initiative is the development of a rapid, accurate, and efficient DNA sequencing technology. A major limitation of current technology is the relatively long time required to perform the gel electrophoretic separations of DNA fragments produced in the sequencing reactions. We demonstrate here that it is possible to increase the speed of sequence analysis by ...
متن کاملDynamic control for ultra-fast separations of organic acids in capillary zone electrophoresis A new direction to improve resolution
Dynamic control and pH changes in the system have been utilized for the separation of twelve organic acids in less than 3 min using capillary electrophoresis (CE). High-speed separations of organic acids under weak acidic conditions indicate the existence of high electroosmotic flow (EOF) caused by treatment of the capillaries with 0.1 M NaOH before each separation. However, strong polyprotic a...
متن کاملA capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution.
Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein-DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (<2 min separations) and simplicity derive...
متن کاملNanomaterials and chip-based nanostructures for capillary electrophoretic separations of DNA.
Capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) using polymer solutions are two of the most powerful techniques for the analysis of DNA. Problems, such as the difficulty of filling polymer solution to small separation channels, recovering DNA, and narrow separation size ranges, have put a pressure on developing new techniques for DNA analysis. In this review, we dea...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Methods in molecular biology
دوره 163 شماره
صفحات -
تاریخ انتشار 2001